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What samples are suitable for Soxhlet extraction method?

2023-03-28
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What is a soxhelt extraction?
The Soxhlet extraction method for extracting crude fat is suitable for samples with high lipid content and low bound lipid content, which can be dried and ground fine, and are not easy to absorb moisture and clump.

Soxhlet extraction method is used for the determination of crude fat content. Fat is widely present in the seeds and fruits of many plants. Measuring the content of fat can be used as an indicator to identify its quality. There are many methods to determine fat content, such as extraction method, acid hydrolysis method, specific gravity method, refraction method, electrical measurement and nuclear magnetic resonance method. At present, the extraction method is widely used both domestically and internationally, among which the Soxhlet extractor method is recognized as a classic method and also a standard method for separating grain and oil in China.



Features of soxhlet extraction.
Easy to operate, easy to extract, and easy to calculate experimental results.

Experimental Principles for soxhlet extraction.
The residual method in Soxhlet extraction is used, which involves reflux extraction with low boiling organic solvents to remove crude fat from the sample. The crude fat content is calculated based on the weight difference between the sample and the residue. Due to the fact that organic solvent extracts contain more or less free fatty acids, sterols, phospholipids, waxes, and pigments in addition to fats, the results determined by the extraction method can only be crude fats.
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